Flow of metabarcoding data in SBDI: A sequencing service provider, such as the National Genomics Infrastructure (NGI), delivers sequencing data to the user. The user submits raw sequencing data and contextual data to the European Nucleotide Archive (ENA), denoises the data using e.g., nf-core/ampliseq, and submits the denoised data (ASVs and their counts in different samples, and contextual data) to the SBDI ASV portal. Metabarcoding data in the ASV database can be searched and downloaded using the portal or using the SBDI4R package (wrapped on the ALA galah package). Figure created by Manash Shah.